Part:BBa_K2888008:Design

fused chimerical gene with 6x His tag (lysozyme and mlrA)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1349
  Illegal AgeI site found at 884
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We try to link two separate gene together. We replace unexpected restriction site with corresponding code

Source

We use common, standard promoter, RBS and terminator. This mlrA gene is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1 and the cyanophage lysozyme was discovered by Heideburg et al in Yellowstone National Park {Heideburg:2009hb}